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NAVICULA SMITHII:

Areolae of this diatom require a n.a. of 0.46 (my 20X S-Plan objective) and green oblique illumination perpendicular to the long axis of the valve face; I resolved them into double rows with a 40X S-plan objective n.a. 0.70, again with oblique illumination.

N. sigma oblique illumination
N. smithii 20X objective oblique illumination

N. smithii oblique illumination
N. smithii 40X objective oblique illumination.